Viva columns are based on a wide pore material we designed for optimal large-molecule separations. In developing Viva silica, we found that although many commercial wide-pore silicas meet the standard 300 Å mean pore size, most have very broad distributions about this mean, with a significant portion of their pore volume falling below 150 Å. This means a large portion of the surface area is unavailable to larger molecules. Viva columns have a narrow distribution around the mean pore size, permitting a larger portion of the silica surface to play a role in the separation.
The Restek PFP Propyl is a great choice for the retention and selectivity of charged bases, electronegative compounds, and amine-containing compounds. Unlike a conventional cyano column, the Restek PFP Propyl is much more amenable to LC-MS because it is more reliable and efficient with acidic mobile phases. This versatile column is also compatible with highly aqueous mobile phases and HILIC separations.
Stationary Phase Category: fluorophenyl propyl (L43)
Ligand Type: pentafluorophenyl propyl
Particle: 5 µm, spherical
Pore Size: 300 Å
Carbon Load: 5%
End-Cap: yes
Surface Area: 100 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C
Features & Benefits
- Excellent for separating peptides or proteins.
- Rugged, spherical particles with 300 Å pore size.
- High proportion of pore/surface area available to large molecules.
Guard column requires Trident LC column protection system or Trident HPLC in-line guard cartridge holder.
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